Substitution of serine caused by a recessive lethal suppressor in yeast.
نویسندگان
چکیده
The SUP-RLl suppressor in the yeast Saccharomyces cerevisiae causes lethality in haploid strains but not in diploid or aneuploid strains that are heterozygous for the suppressor locus. This recessive lethal suppressor acts on amber (UAG) nutritional markers, and can cause the production of approximately 50% of the normal amount of iso-l-cytochrome c in disomic strains that are heterozygous for the SUP-RLl suppressor, and that contain the cycl-179 allele which has an amber codon corresponding to amino acid position 9. The suppressed iso-lcytochrome c contains a residue of serine at the position that corresponds to the site of the amber codon. SUP-RLl was found to lie between thr4 and MAL2 011 chromosome III, approximately 30 map units from the mating-type locus. It is suggested that the gene product of SUP-RLl may be a species of serine transfer RNA that normally reads the serine codon UCG, and that is represented only once in the haploid genome.
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ورودعنوان ژورنال:
- Journal of molecular biology
دوره 102 3 شماره
صفحات -
تاریخ انتشار 1976